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Figure 1 The denaturing high performance liquid chromatography elution profiles for lamin A/C gene (LMNA) mutations detected in three families with dilated cardiomyopathy. (Top) Pedigree of the families: individuals are indicated by generation and pedigree number. Affected status is indicated by filled symbols, unaffected by clear symbols. The (+) and (–) symbols indicate the presence of the mutant allele and the wild type, respectively. (Middle) Elution profiles for the wild-type and patients carrying the LMNA mutations (G266T: III-2; 959delT: II-1; G1130A: II-2; C1718T: II-2). (Bottom) The direct sequencing results illustrate the corresponding nucleotide substitutions (reverse sequence shown for G1130A mutation).