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Figure 6 Exon trapping amplification and sequence analysis of splice product. (A) Reverse transcription-polymerase chain reaction (PCR) amplification products derived from the exon-trapping experiment and revealed on a 1.7% TBE agarose gel. Mutated: PCR fragment corresponding to the exon 22 skipped transcript (209 bp). WT = wild-type transcript (332 bp). (B) Sequence analysis of the 209 bp splice product confirms exon 22 skipping in the mutant allele.