Click on image to view larger version.

Figure 4 Electrophoretic mobility shift assay: allele-specific binding of nuclear protein to the –129C/T polymorphic site. Nuclear extracts were obtained from human umbilical vein endothelial cells after treatment for the indicated time with H₂O₂ (100 µmol/l) or phosphate-buffered saline (PBS) (as a time control). Left panel = interaction with the –129C probe; right panel = interaction with the –129T probe.