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Figure 5 Effect of vitamin C (Vit. C) and anti-LOX-1 monoclonal antibody (LOX-1 Ab) on platelet-induced variations of intracellular nitric oxide (NO) concentration in basal and bradykinin-stimulated bovine aortic endothelial cells (BAECs). The BAECs were preincubated with Vit. C (5 µmol/l) and anti-LOX-1 mAb (30 µg/ml) at 37°C for 30 min. Then the cells were incubated with 10 µmol/l DAF-2 DA at 37°C for 10 min with or without 100 nmol/l bradykinin. Thrombin-activated platelets (3.0 x 10⁷/ml) were added to the BAEC medium for 10 min at 37°C. Results are expressed as mean fluorescence intensity (MFI) and are the means ± SD of experiments performed in triplicate on six separate occasions. ¶p < 0.01, *p < 0.001 vs. control; p < 0.01, p < 0.001 vs. platelets.