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Figure 4 Effect of different amounts of non-activated (T–) and thrombin-activated (T+) platelets on basal and bradykinin-stimulated intracellular concentration of nitric oxide (NO) in bovine aortic endothelial cells (BAECs). The BAECs were preincubated with 10 µmol/l 4, 5 diaminofluorescein diacetate for 10 min at 37°C with or without 100 nmol/l bradykinin. Different amounts of (T–) and (T+) (from 0 to 4.5 x 10⁷/ml) platelets were then added to the BAEC medium for 10 min at 37°C. Results are expressed as mean fluorescence intensity (MFI) and are the means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001, ¶p < 0.01 vs. no addition of platelets (Control).