Click on image to view larger version.

Figure 2 Effect of vitamin C (Vit. C) and anti-LOX-1 monoclonal antibody (LOX-1 Ab) on platelet-induced variations of O2· concentration in bovine aortic endothelial cells (BAECs), Chinese hamster ovary-K1 (CHO) cells, and CHO-K1 cells stably expressing bovine LOX-1 (BLOX-1-CHO) cells. Vitamin C (5 µmol/l) and LOX-1 Ab (30 µg/ml) were preincubated with BAECs, BLOX-1-CHO, and CHO cells at 37° for 30 min. Thrombin-activated platelets (3 x 10⁷/ml) were then added to the cell medium for 10 min at 37°C. Results are expressed as mean fluorescence intensity (MFI) and are the means ± SD of experiments performed in triplicate on six separate occasions. *p < 0.001 vs. platelets alone.