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Figure 6 Terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining (mean ± SE, n = 10) in cardiac myocytes pretreated for 1 h with nicotine (15 ng/ml) versus saline. After 24 h, lipopolysaccharide (LPS) (10 ng/ml) increased TUNEL staining (*p < 0.05). This was attenuated, but not abolished by pretreatment of myocytes with nicotine for 1 h (+p < 0.05).