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Figure 5 Terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining (mean ± SE, n = 10 to 11) in cardiac myocytes isolated from rats pretreated for 7 to 10 days with nicotine (6 mg/kg/day) versus vehicle. In myocytes from saline-pretreated rats, TUNEL staining increased 24 h after lipopolysaccharide (LPS) (10 ng/ml) or angiotensin II (Ang II) (100 nM) compared with vehicle, but only Ang II had an effect in myocytes from nicotine-pretreated rats (*p < 0.05).