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Figure 6 Effect of French red wine on endothelial-type nitric oxide synthase (eNOS) messenger ribonucleic acid (mRNA) stability. The EA.hy 926 cells were preincubated either with ethanol (EtOH-Co, 1.25%, v/v) or with the red wine "Ch.Bonnet reserve" (10%, v/v) for 24 h. To inhibit transcription, actinomycin D (20 µg/ml) was added to the culture medium. Ribonucleic acid was prepared 0, 12, 24, and 36 h thereafter. The eNOS mRNA expression was determined by RNase protection assay. The eNOS mRNA levels in both groups at the time of addition of actinomycin D (0 h) were set at 100%. Circles represent means ± SEM of the densitometric analyses of four different gels. Asterisks indicate significant differences from ethanol-treated cells at the same time points (**p < 0.01; ***p < 0.001; by the Student t test for unpaired data).