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Figure 4 Immunocytochemistry of the right ventricular myocardium in one infant with tetralogy of Fallot, showing the presence of tumor necrosis factor (TNF)-alpha in cardiomyocytes (My), interleukin (IL)-10 in cardiomyocytes, macrophages (M), and endothelial cells (EC), inducible nitric oxide synthase (iNOS) in cardiomyocytes, and nuclear factor (NF)-kappa-B p50 and p65 subunits in the cytoplasma and nuclei of cardiomyocytes. C = negative control (no primary antibody). Magnification: x400 for control, cytokines, and iNOS and x1,000 for NF-kappa-B p50 and p65 subunits. These stains are representative of those obtained in four patients (two in each group).