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Figure 2 (A) Levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-10 measured by Western blotting in the right ventricular myocardium of infants with tetralogy of Fallot (TOF) (solid bar; n = 7) or ventricular septal defect (VSD) (open bar; n = 8). Band intensities for TNF-alpha and IL-10 are normalized for bands of beta-actin. Results are expressed as the mean value ± SEM. *p < 0.01 between groups. (B) Exemplary gels obtained after Western blotting representative of seven experiments in infants with TOF and eight experiments in infants with VSD, showing a higher expression of TNF-alpha but not IL-10 in the former group. The upper panel includes positive controls (PC) (human monocytes stimulated with lipopolysaccharide) and negative controls (NC) (nonstimulated human monocytes). M = marker.