Click on image to view larger version.

Figure 4 Immunohistochemical staining against human leukocyte antigen (HLA)-DR antigens and flow cytometric acquisition of HLA-DR phycoerythrin-conjugated stain in various experimental groups. (A) A coronary section from the infarct-related artery (IRA) (group 1; case no. 1 in Table 1) shows a diffuse positive reaction in the plaque and in the site of cap rupture. A1 shows the flow cytometric results of the same coronary artery after removal of the debris. (B) A coronary section from the non-IRA (same case no. 1) shows a strong positive reaction in the cells through the whole plaque. B1 shows the flow cytometric results after removal of the debris in the same coronary artery. (C) A coronary section from the IRA of a group 2 subject (case no. 22 in Table 1). The positive reaction to HLA-DR is comparable to that in the non-IRA of group 1 (see panels B and Fig. 3). C1 shows the flow cytometric results of the same coronary artery after removal of the debris. (D) A coronary section from group 3 (case no. 30 in Table 1). Only a few cells were positive for the HLA-DR antibody. D1 shows coronary cells from the same coronary artery.