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Figure 1 Acquisition and analysis two-color flow cytometry. Panels A, B, C, D, E, and F represent the control set. (A) Monocytes from peripheral blood cells stained with CD68; the region delimits the area in which positive cells are identified. (B) Smooth muscle cells from the normal tunica media stained with alpha-smooth muscle actin (SMA); the region delimits the area in which positive cells are identified. (C) Lymphocytes from peripheral blood cells stained with CD3; the region delimits the area in which positive cells are identified. (D) Phytohemagglutinin (PHA)-activated peripheral blood cells stained with human leukocyte antigen (HLA)-DR; the region delimits the area in which positive cells are identified. (E) Coronary samples stained with control antibody (mixture of fluorescein isothiocyanate (FITC)/phycoerythrin (PE)-conjugated secondary antibodies). (F) A region gate was drawn to delimit the nonspecific stain. (G) Coronary cells stained with CD3. G1 shows single PE-immunostained cells after debris gating according to control settings. G2 shows the results in the positive region after removal of the debris. (H) Coronary cells stained with CD68. H1 shows single PE-immunostained cells after debris gating according to control settings. H2 shows the results in the positive region after removal of the debris. (I) Coronary cells stained with alpha-SMA. I1 shows single FITC-immunostained cells after debris gating according to control settings. I2 shows the results in the positive region after removal of the debris. (J) Coronary cells stained with HLA-DR. J1 shows single PE-immunostained cells after debris gating according to control settings. J2 shows the results in the positive region after removal of the debris.