Click on image to view larger version.

Figure 2 Effects of calphostin C on translocations of protein kinase C (PKC)- by ischemic preconditioning (PC). The PKC- levels in the cytosol and particulate fraction are expressed as percentages of the total. Ischemic preconditioning significantly reduced PKC- in the cycotol and increased PKC in the particulate fraction, indicating translocation of PKC- from the cytosol to the particulate fraction. In the hearts pretreated with calphostin C (200 nM), translocation of PKC- was not detected. Open bar = cytosol; hatched bar = particulate. *p < 0.05 vs. baseline. n = 4 in each treatment group.