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Figure 5 Oral tolerance with heat shock protein (HSP) 65 attenuates atherogenesis in Mycobacterium tuberculosis and high-fat diet fed low-density lipoprotein receptor deficient mice (LDL-RD). The LDL-RD mice were fed with different doses of HSP65. One day after the last feeding, all mice were immunized with M. tuberculosis and euthanized 12 weeks afterwards. Lesion size was assessed after Oil red O staining of sections obtained at the level of the aortic sinus (A). *p < 0.01; **p < 0.001; ***p<0.0001. In the second experiment, LDL-RD mice were fed different concentrations HSP65, bovine serum albumin (BSA), or phosphate buffered saline (PBS) and fed a high fat diet for four weeks until euthanization. Atherosclerosis was assessed similar to the first experiment (B). *p < 0.01.