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Figure 4 Oral tolerance with heat shock protein (HSP) 65 does not alter IgG isotype distribution. Sera was obtained from all mice tolerized with HSP65 and from non-tolerized mice from experiment 1 and 2. IgG anti-HSP65 isotypes were assessed in the first experiment (A), whereas IgG isotypes (total) were probed in the second experiment (B) employing a capture enzyme-linked immunosorbent assay. Hatched bar = HSP65; open bar = bovine serum albumin (BSA).