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Figure 3 Induction of T helper 2 cytokines by orally tolerized mice. Lymph node cells were obtained from heat shock protein (HSP) 65 tolerized low-density lipoprotein receptor deficient mice immunized with HSP65 upon priming in vitro with 10 µg/ml HSP65 for 48 h. Interleukin-4 (IL-4) levels in the conditioned medium were assessed by a capture enzyme-linked immunosorbent assay kit as described in the Methods section. *p < 0.01.