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Figure 2 In vitro induction of tolerance to heat shock protein (HSP) 65 by co-culture of tolerized splenocytes with HSP65 responder lymph node cells. To assess in vitro tolerization, 10⁵ splenocytes from HSP65 or bovine serum albumin (BSA)-fed (i.e., mice that were fed 5 times with HSP65, 50 µg/dose or BSA) were incubated in the presence of 10⁴ lymph node cells from Mycobacterium tuberculosis immunized mice in the presence of different concentrations of HSP65. The figure represents average values obtained from three studies, each repeated twice. *p < 0.05. Hatched bar = BSA-fed splenocytes; solid bar = HSP-fed splenocytes.