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Figure 4 Evidence for increased nitrotyrosine (NT) formation in hearts from rats with chronic heart failure (CHF). Immunohistochemical staining for NT, an indicator of peroxynitrite formation, in sham (a), CHF (b), and PJ34-treated CHF (c) rat hearts. Panel b shows widespread NT formation in myocytes and intramural vasculature in rats with CHF. Treatment with PJ34 for 10 weeks (c) did not reduce NT formation in rat hearts with CHF. Similar immunohistochemical profiles were seen in n = 4 to 5 hearts per group.