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Figure 3 Examples of flow cytometry data for lipid microbubbles with a net neutral charge with polyetheleneglycol (MB_neutPEG⁺) and lipid microbubbles with a net negative charge without polyetheleneglycol (MB_negPEG^–). Histograms of green fluorescent intensity are shown for microbubbles incubated with serum and florescein isothiocyanate (FITC)-conjugated control monoclonal antibody (mAb) (A), with FITC-conjugated anti-C3b mAb alone without serum (B), or with serum and FITC-conjugated anti-C3b mAb (C). Corresponding fluorescent microscopy images from c samples are shown at the bottom (scale bars = 5 µm).