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Figure 3 Hypoxia/reoxygenation (H/R)-induced Ca²⁺ loading in untreated and 17-ßestradiol (E2)-treated H9c2 cells in the absence and presence of HMR 1098 (3 µM) and 5-hydroxydecanoate (5-HD) (50 µM), sarcolemmal and mitochondrial Adenosine triphosphate-sensitive potassium channel blockers, respectively. (A) Epifluorescent digital images of untreated and E2-treated Fura-2 loaded cells before (left panel) and after H/R (right panel). White bar corresponds to 30 µm. Graphs are time courses of intracellular concentration of Ca²⁺ and correspond to experiments depicted on the left. Each line on graphs represents a single cell from the corresponding image field. (B) Percentage of cells that responded (defined as 50% increase in resting Ca²⁺ with the time-course pattern of Ca²⁺ increase as depicted in A)/did not respond to H/R with Ca²⁺ loading under control conditions (untreated) and 24 h treatment with E2 in the absence (E2) and presence of HMR 1098 (E2 + HMR 1098) and 5-HD (E2 + 5-HD), n = 29 to 45.