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Figure 2 Double-immunostaining for smooth muscle cell alpha-actin (red) and VEGF (brown) of histologic cross-sections of coronary arteries from animals fed a normal diet (N) (A and B) or high cholesterol diet without (HC) (C and D) or with endothelin-type-A receptor antagonism (HC + ET-A) (E and F) for 12 weeks. In contrast with the other two groups, intense vascular endothelial growth factor immunoreactivity is apparent in the outer media and adjacent adventitia in the HC group, including vasa vasorum (arrow in D). Original magnification for (A) 37.5x, for (B) 62.5x, for (C) 62.5x, for (D) 125x, for (E) 62.5x and for (F) 100x.