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Figure 1 Immunohistochemistry demonstrating the distribution patterns of interleukin-6 (IL6), interleukin-6-receptor (IL6R) and glycoprotein 130 (gp130) in donor hearts and controls. Fluorescence visualization of gp130, IL6R and IL6 using rabbit anti-human antibodies and, as secondary antibody/detection system, donkey anti-rabbit conjugated with Texas Red. Control hearts, myocardium of patients undergoing evaluation of ventricular arrhythmias (CTRL) gp130, IL6R and IL6 were not found in control biopsies. Ventricles of donor hearts (DONOR HEART) gp130, IL6R and IL6 occurred in all right ventricular specimens of donor hearts (arrows). Glycoprotein 130 and IL6R were located in interstitial cells, preferentially fibroblasts and myocytes. Interleukin-6 label was mainly detected on myocytes, but also some interstitial fibroblasts were strongly stained. Autofluorescence of the lipofuscin bodies (lf) was evaluated by using the FITC filter (arrowheads = identical distribution patterns). Original magnification: x200.