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Figure 4 Electrophoretic gel mobility shift assay for activation of nuclear factor-B (NF-B). (A) Representative photographs. Lane 1 = reperfused myocardium from wild-type mice; lane 2 = reperfused myocardium from tumor necrosis factor (TNF)- knockout (KO) mice; lane 3 = reperfused myocardium from wild-type mice with competitive oligonucleotides. (B) Summarized data on activation of NF-B in wild-type mice (n = 5) and TNF- KO mice (n = 5). *p < 0.01.