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Figure 3 Effect of N-2-mercaptopropionyl glycine (MPG) on heme oxygenase (HO)-1 mRNA expression. Total RNA was isolated from hearts treated with (+) or without (–) MPG and analyzed for expression of HO-1 by northern blot. A representative northern blot analysis result is shown. Sham = sham-operated mice; TAC = transverse aortic constriction (TAC)-operated mice. Lower panel shows the quantitative analysis of HO-1 mRNA expression relative to ß-actin mRNA based on densitometric measurements on northern blot. Data are expressed as mean ± SEM (n = 3). Control Sham = vehicle-treated sham-operated mice; control TAC = vehicle-treated TAC-operated mice; MPG sham = MPG-treated sham-operated mice; MPG TAC = MPG-treated TAC-operated mice. Two-way analysis for variance (ANOVA) was used to test significance, with one factor being sham versus TAC operation and the other factor being vehicle versus MPG treatment. As we faced a significant interaction, we have analyzed the data among groups by one-way ANOVA with Tukey-Kramer’s post hoc test.