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Figure 5 The effect of nitroglycerin (NTG) on tissue inhibitor of metalloproteinase (TIMP)-1 expression in human monocyte-derived macrophages. Monocyte-derived macrophages were exposed to control media, NTG 200 pmol or NTG 2,000 pmol for 4 h. Ribonucleic acid was extracted and competitive reverse transcription-polymerase chain reaction performed to measure TIMP-1 messenger ribonucleic acid (mRNA) levels (solid bars) Y-axis represents mRNA levels as % of control. Aliquots of conditioned media were collected and TIMP-1 protein levels measured by enzyme-linked immunosorbent assay (open bars). Y-axis represents protein concentration (ng/ml). Results represent the mean ± SEM for analysis of three independent experiments. **p < 0.005 compared with control cells.