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Figure 3 Gelatin zymogram in balloon angioplasty (BA)-treated and stent arteries. Conditioned medium from chondrocyte cultures were run as positive controls to show 92 kD and 72 kD gelatinolytic activity, which was confirmed by molecular weight markers (not shown). In both BA-treated and stent arteries, 72 kD and 62 kD gelatinolytic bands are evident, corresponding to the pro- and active forms of matrix metalloproteinase (MMP)-2. In stented (but not BA-treated) arteries, an additional gelatinolytic band at 92 kD is evident, corresponding to MMP-9 activity.