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Figure 6 4-Aminopyridine (4-AP) phosphorylates proline-rich tyrosine kinase 2 (Pyk2) (A) and epidermal growth factor receptor (EGFR) (B) in cardiomyocytes. Cardiomyocytes were stimulated with 4-AP for the indicated times, and the tyrosine phosphorylation of Pyk2 and EGFR was detected by immunoprecipitation-Western blot analysis. Endothelin (ET-1) was used as a positive control agent. Anti-p-Tyr represents anti-phosphotyrosine antibody. 4-Aminopyridine induced phosphorylation of Pyk2 and EGFR at 5 and 10 min, respectively, in cardiomyocytes.