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Figure 4 (A–C) Representative histograms of intraplatelet nitrotyrosine production in a nonsmoker and a smoker. In the smoker before vitamin C administration, the fluorescent peak of nitrotyrosine in platelets stimulated by collagen (3 µg/ml) (open area) shifted to the right from fluorescent peaks of unstimulated platelets (solid area). In the smoker after vitamin C administration, the histograms were identical to those of the nonsmoker. (D) Intraplatelet nitrotyrosine production by collagen-induced platelet aggregation in nonsmokers (n = 8) and in smokers (n = 8).