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Figure 4 (A) Synthesis of tumor necrosis factor- (TNF) and interleukin-10 (IL10) detected in the myocardium 6 h after cardiopulmonary bypass (CPB) by Western blot in pigs that were in moderate hypothermia during surgery (temperature [T°]: 28°C) (solid bar) and in normothermia (T°: 37°C) (open bar). Band intensities for cytokines were normalized for bands of ß-actin. Results are shown as mean ± SEM. *p < 0.05 between groups, respectively. (B) Exemplary gel obtained after Western blot representative for six independent experiments in each group (moderate hypothermia: T°, 28°C and normothermia: T°, 37°C) showing the effect of core T° during CPB on TNF and IL10 synthesis. C = control sample before CPB.