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Figure 3 (A) Gene expression of tumor necrosis factor- (TNF) and interleukin-10 (IL10) detected in the myocardium taken 6 h after cardiopulmonary bypass (CPB) by reverse transcript polymerase chain reaction in pigs that were in moderate hypothermia during surgery (temperature [T°]: 28°C) (solid bar) and in normothermia during surgery (T°: 37°C) (open bar). Results are expressed as the ratio of cytokines messenger RNA (mRNA)/ß-actin mRNA and are shown as mean ± SEM. *p < 0.0001 and p < 0.1 between groups, respectively. (B) Exemplary gel representative for six independent experiments in each group (moderate hypothermia: T°, 28°C and normothermia: T°, 37°C) showing the effect of core T° during CPB on TNF and IL10 gene expression. Lower panel shows corresponding 18S and 28S ribosomal RNA bands as loading control. C = control sample before CPB; M = molecular-weight marker.