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Figure 5 Ischemia-reperfusion-induced Ca²⁺ loading in male and female cardiomyocytes. Typical time course of Fura-2 fluorescence ratio (left and center panels) and average concentration of intracellular Ca²⁺ at rest and after ischemia-reperfusion (right panel) obtained with cardiomyocytes isolated from male and female guinea pigs. Bars represent mean ± SEM (n = 8 to 10). *p < 0.05 when compared with its own control (graph) or when compared with the same condition in the opposite gender (graph).