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Figure 5 Effects of phenylephrine (PE) treatment (14 days) on myocardial gene expression in nontransgenic littermate control (NLC) mice and transgenic mice with cardiac targeted overexpression of the wild-type _1B-AR (Tg43). (A) Representative Northern blot analysis of the ventricular transcript level of atrial natriuretic factor (ANF), skeletal -actin, sarcoplasmic reticulum Ca⁺⁺-ATPase (SERCA2a) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in the listed mice phosphate-buffered saline and PE treated. (B) Pooled quantitative data for the individual genes shown in A. Histograms represent the mean ± SEM of quantified Northern blots using the ribonucleic acid isolated from three left ventricles in each group. The data were normalized to the levels of hybridization of GAPDH and plotted as foldover GAPDH levels. *p < 0.05 versus sham-treated NLC mice.