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Figure 1 Shown are representative autoradiographs used to measure the relative abundance of transcripts in RNA isolated from vastus lateralis muscle at baseline and after 12 weeks of training. Reverse transcription reactions included either 0.25 µg (1x) or 0.5 µg (2x) total RNA. Polymerase chain reaction conditions were chosen to ensure that product derived from the 2x sample was doubled compared with the 1x sample, demonstrating that the assays are at least semiquantitative. Aliquots of the same reverse transcription reaction for each patient were used for each polymerase chain reaction using primers specific to the various gene products. Cu/Zn SOD = copper zinc superoxide dismutase; eNOS = endothelial nitric oxide synthase; GSH-Px = glutathione peroxidase; VWF = Von Willebrand factor.