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Figure 2 (A) Representative experiments showing upregulation of Na+-HCO3– symporter isoform-1 (NBC-1) messenger RNA (mRNA) in the myocardium of sham-operated and placebo-treated animals with myocardial infarction (MI) one day and seven days after MI. Total RNA obtained from the left ventricular (LV) free wall was amplified by reverse transcription polymerase chain reaction (upper line) and analyzed by Northern blot (lower line). (B) Relative changes in mRNA expression were determined by densitometric analysis and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) signals. *p < 0.05 compared with sham operation. Data represent mean ± SEM, n = 6.