Click on image to view larger version.

Figure 2 Effects of diazoxide and EIPA on mitochondrial flavoprotein fluorescence and I_K.ATP. Upper panel: although diazoxide (100 µM) induced oxidation of mitochondrial flavoprotein, EIPA (1 µM) did not provoke significant mitochondrial oxidation. Ethylisopropyl amiloride did not enhance mitochondrial oxidation by diazoxide. The flavoprotein fluorescence was calibrated by exposing the cells to 2,4-dinitrophenol (100 µM). Lower panel: data for I_K.ATP measured at 0 mV. Neither diazoxide nor EIPA increased I_K.ATP. EIPA = ethylisopropyl amiloride; I_K.ATP = sarc-K_ATP channel current.