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Figure 3 A, Representative Northern blot analysis of eNOS mRNA expression after exposure of human neutrophils to TNF-alpha (500 U/ml) for different periods. The experiments were performed in the presence of the transcriptional inhibitor DRB (60 µmol/liter). B, The graph shows the densitometric value of the eNOS hybridization signal relative to glyceraldehyde-3-phosphate dehydrogenase, expressed as a percentage of the control value in the absence of TNF-alpha. The GAPDH probe was used as a loading control for loading. *p < 0.05 vs. 0 h. DRB = 5,6-dichloro-1-2-D-ribofuranosylbenzimidazole; eNOS = endothelial-type nitric oxide synthase; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.