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Figure 2 Representative Western blot analysis demonstrating the expression of eNOS protein in TNF-alpha–incubated (500 U/ml) human neutrophils. Human neutrophils were incubated for different periods with TNF-alpha (500 U/ml). (Densitometric analysis in arbitrary units—0 h: 100; 2 h: 117 ± 8; 4 h: 108 ± 9; 6 h: 60 ± 7*; 8 h: 23 ± 8*; [n = 6];*p < 0.05 vs. 0 h.) eNOS = endothelial-type nitric oxide synthase.