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Figure 2 Experimental protocols for study 2. All groups were equilibrated for 30 min in aerobic conditions (37°C). Following this, the right atrial slices were randomly assigned to one of the following study groups (n = 6/group): Aerobic Control = aerobically incubated for the entire experimental time course. Ischemia Alone = subjected to 90 min simulated ischemia followed by 120 min reoxygenation. PC = preconditioning with 5 min ischemia followed by 5 min reoxygenation before undergoing 90 min ischemia. Diazoxide = incubation in 0.1 mM of the drug for 10 min before exposure to 90 min ischemia.