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Figure 1 Experimental protocols for study 1. All groups were equilibrated for 30 min in aerobic conditions (37°C). Then the right atrial slices were randomly assigned to one of the study groups (n = 6/group): Aerobic Control = aerobically incubated for the entire experimental time. Ischemia Alone = subjected to 90 min simulated ischemia followed by 120 min reoxygenation. PC = preconditioning with 5 min ischemia followed by 5 min reoxygenation before undergoing 90 min ischemia. Diazoxide = incubation in 0.1 mm of the drug for 10 min before exposure to 90 min ischemia. Glibenclamide = pretreatment in 10 µm of the drug for 10 min before preconditioning was applied.