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Figure 3 Reduction of whole-cell I_Ca by the postischemic coronary effluent. A, I_Ca recorded from a rat ventricular myocyte before (control; solid circles) and 1 min after adding 1:4 diluted postischemic effluent to the superfusate (open circles). The time-dependent decay of I_Ca was matched to a double-exponential function (thin solid line; time constants = 4 and 20 ms). B, Plot of peak I_Ca as a function of the test-step potential (iv curve) before (control; filled circles) and after adding 1:4 diluted postischemic effluent to the superfusate (open circles). The holding potential was –45 mV. Between –40 and 50 mV, voltage dependence of peak I_Ca is matched, as calculated from the Boltzman formula. Effluent = postischemic coronary effluent (dilution 1:4).