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Figure 2 In situ expression of TnC in injured rat arteries. TnC mRNA was detected by in situ hybridization 3 (A) or 14 (B) days after balloon injury of rat carotid artery using full-length TnC riboprobes. Cryosections were treated with proteinase K. After hybridization, the sections were washed, dried, coated and exposed in the dark at 4°C for four weeks. After development, the sections were counterstained with hematoxylin and eosin to aid in cell identification and viewed on a photomicroscope. TnC = tenascin-C.