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Figure 1 Immunohistochemical localization of TnC in rat carotid arteries. Frozen sections of uninjured (A) as well as arteries collected two days (B), three days (C), four days (D), five days (E) and seven days (F) after injury were stained with a polyclonal anti-TnC antibody (1:500). Adjacent sections were stained with control preimmune antibodies. The lumen is toward the top of each panel. All photomicrographs are at a x100 magnification. Substitution of anti-TnC antibodies with an equivalent concentration of preimmune rabbit serum (1:500) or an irrelevant antibody produced no staining of normal or injured carotid artery sections. adv = adventitia; m = media; TnC = tenascin-C.