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Figure 1 Experimental protocols for eight experimental groups. All protocols were preceded by a stabilization period of 45 to 60 min. This was followed by 90 min of simulated ischemia (superfusion with hypoxic, substrate free Modified Tyrodes solution and pacing at 3 Hz) and 120 min of superfusion with reoxygenated Tyrodes solution. Control = C; preconditioning = PC; delta opioid agonist = DADLE; naltrindole + preconditioning = naltrindole + PC; 5-hydroxydecanoate = 5HD. Solid box = simulated ischemia; open box = normoxic superfusion.