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Figure 2 Nasal administration of CM suppressed production of IL-2, TNF-, and IL-1ß by splenocytes in response to CM. Upon sacrifice on day 21, the spleens were collected from mice that received cardiac myosin intranasally (right bars, n = 9) and those that received vehicle buffer (left bars, n = 14). The splenocytes were cultured in vitro in the presence of either CM (10 µg/ml) or concanavalin A (ConA). The levels of cytokines produced in the culture supernatant were measured after 48 h and presented as the mean of each group ± SEM. The levels of IL-2 (p = 0.048), TNF- (p = 0.04), and IL-1ß (p = 0.02) produced in response to CM were significantly decreased in mice that received CM intranasally, compared to those that received buffer intranasally. The levels of IL-2 produced in response to ConA were not reduced after nasal instillation of myosin.