Click on image to view larger version.

Figure 5 Effects of cerivastatin on cell-cycle regulatory proteins: Western blotting demonstrated that stimulation of human vascular smooth muscle cells with PDGF-BB (10 ng/ml) for 24 h induced activation of Cdk2, phosphorylation of pRb and down-regulation of p27^Kip1, but not p21^Cip1. Cerivastatin (10^–6 mol/liter) inhibited Cdk2 activation and pRb phosphorylation, but did not prevent the down-regulation of p27^Kip1. The p21^Cip1 was also not influenced by cerivastatin. The effects of cerivastatin on Cdk2 and Rb were reversed by the HMG-CoA product mevalonate (2 x 10^–4 mol/liter). The results were repeated with three independent experiments.