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Figure 6 The roles of MEK-1 and PI3'-kinase in the ET-1-mediated inhibition of apoptosis. Cardiac myocytes were cultured in serum-free media in the presence or absence of Iso (10^–5 mol/liter), ET-1 (10^–7 mol/liter), PD098059 (an MEK-1-specific inhibitor), wortmannin (PI3'-kinase inhibitor), or rapamycin (pp70 S6-kinase inhibitor) as indicated for 48 h. The % TUNEL-positive nuclei values were calculated by counting an average of 400 to 500 nuclei in each slide. Values are mean ± SE of four independent experiments.