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Figure 5 Complete inhibition by ET-1 of 8-Br-cAMP-induced apoptosis. Cardiac myocytes were cultured in serum-free media in the presence or absence of 8-Br-cAMP (3 x 10^–2 mol/liter) and ET-1 (10^–7 mol/liter) as indicated for 48 h. The % TUNEL-positive nuclei values were calculated by counting an average of 400 to 500 nuclei in each slide. Values are mean ± SE of two independent experiments.