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Figure 4 Movat’s stain. (A) Iliac artery of cholesterol-fed rabbit 60 days after balloon injury and local saline (control) application. Cross-section shows representative fibrocellular hyperplasia with focal macrophage/foam cell accumulation in the core. Waviness of both remaining internal and external laminae is suggestive of a remodeling process leading to shrinkage. (B) Contralateral iliac artery of the same animal after balloon injury and local application of glue. Cross-section shows comparable initial mechanical injury with fibrocellular hyperplasia without significant foam cell accumulation. Little inflammation and no angiogenesis are seen, but the tunica adventitia displays some reinforcing collagenous thickening in the upper left quadrant. (C) and (D) Iliac arteries from another cholesterol fed animal 60 days after procedure. On both iliac there is neointima around the entire circumference, but the internal elastic lamina is intact, and the original tunica media shows intimal cell loss and little compression, indicating that in these positions the initial injuries were present, symmetric and comparable. (C) The segment treated with endothelial cell seeding shows a concentric lesion with the fibrocellular intimal hyperplasia. (D) Local treatment with glue/cell reconstruction results in a minimal fibrocellular hyperplasia with little variation in thickness mostly due to the previous neointima. (A) to (D) Movat stain all taken with a digital camera at the same magnification and further processed with photofinish 4. L = lumen; M = media; N = neointima.