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Figure 2 Immunohistochemical analysis for TNF-alpha and TACE in serial endomyocardial biopsy tissues obtained from patients with myocarditis. Detection of TNF-alpha (a) and TACE (c) proteins with primary and secondary antibodies in the initial biopsy. TNF-alpha and TACE proteins were found in myocytes, infiltrating macrophages and mononuclear cells. Immunostaining of TNF-alpha (b) and TACE (d) was markedly decreased in myocytes and interstitial cells in repeat biopsy. Magnification for a and c, x230; for b and d, x240, reduced by XX%.